Let's witness what Max has to reveal on the subject of ?Basketball Rundowns. It's just at the moment that people are beginning to recognize about Recreation and Sports, talking about it on much bigger magnitude. In the past this matter was not that talked about. Essentially as individuals linked with the subject did not do enough. It is greatly recommended that you take your time reading through this write-up instead of speed reading it, which might lead to some missed points.
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As the head of a basketball team, or as a member; there are certain extra pieces of putting together a team that are important to remember. The game of basketball is more than preparing for a game or going to shoot some extra hoops. There are also several other approaches that can be used in order to help build the team and allow them to play their best.
The basics of making sure that basketball works for the entire team are to make sure that the right training is done. Most coaches will require that conditioning is done every day in order to keep the team in shape for the game. Because of the high level of physical intensity on the court, it is best that the team knows how to move properly. This includes practicing running on the court, to conditioning the team to shoot, dribble and work as a team both offensively and defensively.
Warning: Whatever thing within this article is individual view of its author on "?Basketball Rundowns", you have to do your own study to come up with accurate facts. Proper consultant on "?Basketball Rundowns" should be consulted before taking any action. OvalEgg.com is not responsible for any damages of any kind that may arise from applying information contained in this article.
Of course, while conditioning and while one is in a game, injuries are likely to occur. It is not uncommon for one to fall while they are moving or to be pushed over by an opponent. You will want to make sure to remember who has had an injury, check on how well it is healing and to not push or strain the injury during practice or on the court. Taking care of the players is an integral part of playing the game properly.
Basketball isn?t just about the physical needs that need to be met for every game. It is also about having a mental attitude that is dignified when playing the game. When one is playing a game, or practicing, there need to be certain behaviors that are enforced. Respect for other players and the coach, dignity about the rules, and other types of attitudes may not win the game but will help the players to walk away with an understanding of what it takes to feel good after every game.
If you want to make sure that your team is playing the game of basketball properly, you don?t want to stop on giving them the basics of how the game is played. Basketball is a game that calls for action by team members that can work together and understand how to win effectively. If you are coaching a team, you want to make sure that all of the needs of the team are met so they can walk onto the court with the right mindset.
Thanks for taking a look at this peace of writing from Max. If you are planning to take this article for your use in any way, please link back to this page. I hope you appreciated what I wrote on "?Basketball Rundowns". I would be pleased to know what you think concerning it, negative or positive. Please leave a comment below and show me you're alive.
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KIRKUK, Iraq (Reuters) ? A suicide bomber killed three people and wounded dozens in Iraq's Kirkuk city on Thursday after he blew up his car outside a bank where police were collecting their salaries.
The bomber drove his car into the bank premises, damaging nearby buildings and setting parked vehicles on fire in the city center, local authorities said.
Black smoke billowed from the blast site as emergency workers helped the wounded into ambulances. One body lay in the street near the wreckage from the attack.
Kirkuk, 250 km (155 miles) north of Baghdad, is ethnically divided among Iraqi Arabs, Kurds, and Turkmen and its disputed status makes it a potential flashpoint for conflict as U.S. troops prepare to withdraw at year-end.
"The security measures around the bank were tight but the suicide bomber stormed the bank by car and managed to blow himself up although security force opened fire and injured him," Hassan Turan, head of Kirkuk provincial council, told Reuters.
"The attack targeted the bank because it gives salaries to governmental offices," he said.
Three people were killed and 76 others wounded, mostly local residents, said Sideeq Omar, general director of Kirkuk health department.
Violence has fallen sharply in Iraq since the sectarian strife in 2006-2007, but Sunni Islamist insurgents tied to local al Qaeda affiliates and Shi'ite militias still carry out daily bombings and assassinations.
Attacks this year have often targeted local government offices or Iraqi security forces as insurgents have tried to destabilize Prime Minister Nuri al-Maliki's power-sharing government, split among Shi'ite, Sunni and Kurdish blocs.
Around 44,000 U.S. troops remain in Iraq more than eight years after the invasion that toppled Saddam Hussein, but they are scheduled to leave by the end of the year when a bilateral security agreement ends.
Iraqi and U.S. officials believe local armed forces can contain any internal threat now, but Baghdad and Washington are in talks about whether some U.S. troops would stay beyond 2011 as trainers to help Iraq cover gaps in its capabilities.
(Reporting by Mustafa Mahmoud and Muhanad Mohammed, Writing by Patrick Markey; Editing by Karolina Tagaris)
Some great baseball achievements will stand the test of time
The Washington Post / Washington Post via Getty Images
Cal Ripken Jr. takes a victory lap after playing in a record 2,131st?straight game on Sept. 6, 1995. He would add another 501 games to his record before finally taking a day off in 1998.
OPINION
By Bob Harkins
NBCSports.com
updated 11:03 a.m. ET Sept. 27, 2011
"I'm hoping someday that some kid, black or white, will hit more home runs than myself. Whoever it is, I'd be pulling for him." ? Hank Aaron
It was April 8, 1974, when Hank Aaron flicked his wrists and sent the ball sailing over the left-field wall.
It was something he had done hundreds of times before, but this one was special. It was the 715th home run of his career, and it had broken the most-hallowed of all baseball records, a record held by the legendary Babe Ruth, a record that had stood for 39 years.
It was a mark some thought would never be broken, having been unchallenged for so long. Before Aaron, only one player had even managed to give it a serious run, and that player ? Willie Mays ? finally ran out of gas with 660 homers in 1973.
Aaron would go on to add 40 more home runs to his record, retiring in 1976 with 755. An amazing example of consistency and longevity, Aaron had to average 33 home runs a season over his 23-year career to set the record. If not hopelessly out of reach, his record certainly seemed secure.
Then again, Babe Ruth fans probably thought the same. They couldn?t have predicted Aaron would come along.
And then again, who could have predicted Barry Bonds?
Bonds, the surly slugger whose father Bobby was a contemporary of Aaron?s, brought an incredible combination of raw power, dazzling bat speed, and a surgeon?s eye to the game. Sparked by his amazing 73-homer season in 2001 (another record), he ended Aaron?s home run reign in 2007, and finished his career with 762.
There are plenty of questions about how Bonds attained the home run record, with many people believing he used illegal performance-enhancing drugs. (In his trial this year, a jury couldn?t decide if Bonds lied to a grand jury about his drug use, though he was convicted of obstruction of justice in the case.)
But regardless of how Bonds got there, the record remains. Now the only question is will anyone ever break it?
?Seventy-three home runs will probably never be done again,? says Cincinnati Reds first baseman Joey Votto, a two-time All-Star with 118 home runs in five seasons, ?and the (career) home run record will probably never be done again, at least in the next 100 years.?
Maybe so, but as Bonds? proved, you never know for sure.
Records are made to be broken, the old saying goes. Babe Ruth found out the saying was true, and Hank Aaron predicted it would be, but is that really the case?
Let?s take a look at some of the other great achievements in baseball, and with the help of input from the players themselves, attempt to predict which marks will fall, and which will stand the test of time.
CAL RIPKEN JR.?S STREAK OF 2,632 CONSECUTIVE GAMES PLAYED
Imagine going 17 years without missing a single day of work. Now Imagine your job description includes turning double plays as base-runners try to knock you on your backside, taking countless sprints around the bases, and dodging the occasional 95-mph fastball aimed at your ribs, knee, elbow, and hands. Now you have an idea what Cal Ripken Jr., who spent most of his career at the demanding position of shortstop, had to go through to break Lou Gehrig?s record.
?In baseball now, you play so hard,? says Texas Rangers third baseman Adrian Beltre. ?You can pull a hamstring, you can sprain your wrist. There are so many things that go against even being close to that record.
?Even if you do stay healthy, you get tired. To be in the lineup every day, 162 games, is almost unheard of. You might last a couple years, but not 12 or 14 years.?
Ripken broke Gehrig?s 56-year-old record of 2,130 in 1995, then proceeded to add another whopping 501 games to his total.
To put it in perspective, only seven players have even played more than 1,000 games in a row, and the man in third place all-time ? Everett Scott ? compiled fewer than half as many as Ripken (1,307).
?It?s amazing what Cal Ripken did,? says Atlanta Braves catcher Brian McCann. ?I can?t even put into words how hard that is. To never have anything go wrong. Luck was on his side and he had the determination to go out there and perform every day. You put those things together and it?s amazing what he did.?
Verdict: Will anyone break Ripken?s record? ?I don?t see it happening,? says Beltre. We agree.
JOE DIMAGGIO?S 56-GAME HITTING STREAK/HITTING .400
We?ve included these two together because it?s been 60 years since either happened. It was 1941 when Yankees great Joe DiMaggio set his record. DiMaggio?s rival, Boston Red Sox legend Ted Williams, hit .406 that same year, and neither mark has been touched since. A Yankee and a Red Sox. Two amazing achievements that haven?t been matched in 60 years. It just seems right to keep them together.
Keystone / Getty Images
Hall of Famer Joe DiMaggio hit safely in 56-straight games in 1941.
There have been some close calls. Pete Rose compiled a 44-game hitting streak in 1978, and Paul Molitor reached 39 in 1987. In the chase for .400, Tony Gwynn hit .394 in 1994, and Rod Carew hit .388 in 1977. But that?s about it.
There are a number of reasons that will make it difficult for anyone to top either record, including an increasing reliance on specialist relievers out of the bullpen and the intense focus of attention from the modern-day 24-hour news cycle, neither of which DiMaggio or Williams had to face.
?If Ichiro can?t get it, nobody can get it,? says Votto, describing the type of player it would take to break DiMaggio?s streak. ?You can?t be a guy who walks, you can?t be a guy that strikes out. You can?t be a guy who is too deep in the order, because you?ll lose opportunities. And with the way the bullpens work now, if you?re at game 40, you?re facing some specialists-times-two, innings six-through-nine, for your last two at-bats. Good luck, you know??
Beltre agrees. ?It would take a contact hitter, a hitter kind of like Ichiro. A guy who can get a dribbler for a hit, who can get a blooper for a hit, a guy who doesn?t walk a lot, but he can get a hit any time.?
As far as hitting .400 goes, it would likely take the opposite kind of hitter, the kind who has a more discerning eye at the plate and is willing to be patient and wait for a good pitch to hit.
?It would take a lot of walks, a lot of walks, and a lot of walks,? says Cincinnati Reds second baseman Brandon Phillips. ?You gotta make sure you get at least two hits, or one hit and go 1-for-2. You gotta get two hits, or one hit and a lot of walks. Then you could do it.?
Verdict: Either mark could be reached under the right circumstances, but the planets would have to align in an almost unprecedented way. As Votto says, ?the pressure media wise and the overall attention will be exponentially more than anything Joe DiMaggio dealt with or Pete Rose dealt with. It?s too much.? That?s probably a big reason neither mark has been touched in 60 years.
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Down to the wire
??HBT Extra: Craig Calcaterra gives Tiffany Simons his predictions as the NL and AL wild-card races go down to the final night of the season.
Jim Rogash / Getty Images
HBT: 4 teams, 2 playoff spots, 1 exciting day
HBT: The final day of baseball season certainly won't be lacking in drama. Tied with the Rays, the Red Sox put their faith in Jon Lester as they try to avoid an embarrassing collapse. In the NL, the Braves aim to hold off the hard-charging Cardinals.
WASHINGTON (Reuters) ? The chances of the economy averting a new recession got a boost on Thursday with claims for jobless benefits falling to a five-month low last week and growth a touch stronger in the second quarter than previously estimated.
Initial claims for state unemployment benefits fell 37,000 to 391,000, the Labor Department said, well below economists' expectations for 420,000. But the department cautioned that the way it adjusts the data for seasonal fluctuations may have overstated the strength.
Separately, the Commerce Department said U.S. gross domestic product grew at an annual rate of 1.3 percent in the second quarter, up from the previously reported 1.0 percent. Consumer spending and export growth both were stronger than earlier estimated.
"When you connect these data points together, they indicate a very tepid recovery. We are still experiencing positive growth, which is better than we feared a few months ago," said Paul Ballew, chief economist at Nationwide in Columbus, Ohio.
The cautious optimism generated by Thursday's data was tempered somewhat by a report showing the housing sector remained on the rocks last month.
Stocks on Wall Street were mostly higher on the data and growing confidence in Europe's response to its debt crisis. The dollar fell against a basket of currencies, while prices for U.S. government debt dropped.
FED READY TO ACT AGAIN
Political haggling in Washington over budget policy and the deepening debt crisis in Europe had eroded confidence, leaving the U.S. economy on the brink of a new recession.
A survey of U.S. chief executives released by the Business Roundtable on Thursday showed their views of the economy's prospects deteriorated in the third quarter, with the number who expect to cut jobs roughly doubling.
Faced with a weak recovery, the Federal Reserve last week announced a new measure designed to push long-term borrowing costs lower by shifting assets on its balance sheet.
In speech on Wednesday, Fed Chairman Ben Bernanke said the U.S. central bank might need to ease monetary policy further if inflation or inflation expectations fell significantly.
The drop in initial claims for unemployment benefits took them below 400,000 for the first time since early August. The department, however, said the weakness in the labor market in recent years may have led the model it uses to seasonally adjust the data to overstate last week's drop.
The decline also reflected the fading impact of Hurricane Irene, which had caused claims to spike in the September 10 week.
Still, the total number of unemployed continuing to claim benefits after an initial week of aid fell to 3.73 million in the week ended September 17 from 3.75 million a week earlier.
The September 17 week corresponds with the survey period for the department's household employment measure, which is used to construct the national unemployment rate.
RECESSION FEARS EASE
In August, the jobless rate remained stuck at 9.1 percent, with a separate survey of employers showing hiring ground to a halt, which ratcheted up recession fears.
Those worries are beginning to fade. Factory output continues to expand and businesses have maintained their appetite for spending on capital goods.
"Indications are that the third quarter is doing better than previously thought. We now anticipate third-quarter real GDP growth of just above 2 percent," said Nigel Gault, chief U.S. economist at IHS Global Insight in Lexington, Massachusetts.
Housing, however, remains a weak spot.
The National Association of Realtors said its index of pending home sales, based on contracts signed in August, fell 1.2 percent to 88.6, its lowest since April.
NAR said contract signings, which usually precede actual closings by a month or two, were held back by tight credit and, in the Northeast, by Hurricane Irene.
With millions of Americans locked into mortgages worth more than their homes, historically low interest rates are failing to lift sales. Freddie Mac said on Thursday that the average rate on U.S. 30-year fixed rate mortgages fell to a record low 4.01 percent this week.
(Additional reporting by Jason Lange, Editing by Andrea Ricci)
A lot of imagine that zit issues are temporary and might be simply outgrown. Depressing to say, most victims do not actually get past their teenage years. The lack of fine pimples remedy along with a complete skincare solution evaluation often qualified prospects many pimples victims to breakdown mentally and emotionally. Some even ponder suicide in accordance to some current clinical investigation.
Prior laboratory tests showed that the danger to possessing psychological issues that often result in bodily harming one?s self is connected to different pimples remedy and therapies. Other researchers sustain that significant sorts of this skin situation intrinsically result in depression with or without any aggravating result from medications.
Surveys show that teenage ladies and boys with severe pimple issues are 2 times and thrice probable to ponder suicide than counterparts with fairer complexion. They may be over 2 times as probable to lack friends. Victims are also anticipated to never knowledge intercourse and do inadequately at school. Actually, youngsters with severe zit conditions are located to participate significantly less in lots of pursuits these as sports activities and workouts, which could additional complicate their conditions. Reduced ranges of bodily exercise also raise the danger of getting health-related issues these as heart condition and diabetes later on in lifestyle.
People who are extremely anxious about their skin situation are apt to consider on their own inferior and may knowledge a poorer quality of lifestyle. This phenomenon transpires routinely to the two males and females. Professionals say that dermatological social anxiety is frequently ignored in lots of scientific studies as an important motivating factor for sport and workouts. The dread of getting one?s skin evaluated by their friends are considerable implications for bodily and social well-being.
If you?d like to this oil to deal with your pimples, you don?t desire to wash your confront using the stuff. Simply dab a modest quantity around the blemishes and allow it sit.
I generally recommend applying it at nighttime ahead of bedtime and then allowing it sit around the skin overnight. When you wake up within the morning, you ought to recognize a significant reduction within the blemishes.
That is on the list of only topical pimples treatment options that I?ve noticed operate on large and painful pimples blemishes. Open up up your pores with some steam and then apply the tea tree oil. Let it sit overnight and the discomfort and inflammation ought to subside instead rapidly.
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Flooding struck Five Points on Sept. 25, 2011.
Columbia, SC (WLTX) ? News19 is On Your Side with the answers to frequently asked flooding coverage questions from South Carolina Insurance News Service officials.
Russ Dubisky with SCINS provided the following answers to News19?s questions:
News19: If your car floods in your driveway, who is responsible for coverage (homeowners or car insurance)??
Dubisky: If your vehicle floods, damages will be covered by your auto policy.
Do you need special flood insurance for your vehicle like you do for your home??If your car is parked on a street and is damaged by flood waters are you covered?
While no ?special? flood policy is required to cover flooded automobiles, you will need comprehensive coverage, which is separate from the state required liability coverage. The same comprehensive coverage is required if your vehicle were covered on the side of a road or in a parking lot, or driving through rushing waters.
If your home floods because of a leak in the roof during heavy rain, do you need flood insurance to cover the repairs?
Flood could generally be defined by rising water that damages a home. If the water damage comes from above (like a hole in your roof) the homeowners? policy would cover the water damage, and no flood policy would be needed in that instance.?
If a tree is uprooted in your neighbor?s yard and damages your home, whose insurance is responsible for coverage?
If your neighbor?s tree falls and damages your house, the claim would typically be made to the insurer of the damaged home. However, if there is a history of the neighbor asking to trim or remove a dead tree before the incident, there may be exceptions and the property from which the tree fell could be held responsible.
Will insurance pay to remove trees that could potentially cause damage to your property because they are diseased or could fall during a storm?
Tree removal is considered a part of basic home maintenance and would not be covered by a homeowners? insurance policy unless the fallen tree damages insured property (garage or home).
Self esteem is nothing but what we analyze about ourselves and many individuals have low self esteem about themselves and there are self esteem self help tips and techniques to help them improve self esteem. Having poor image of yourself and not believing in your abilities and capabilities generally results in low self esteem. Boosting self esteem would help an individual develop confidence and provide the strength to face the challenges of life without difficulty. Good and effective self esteem self help tips and techniques would help them develop communication and develop relationships with their loved ones. Read this article to learn various self esteem self help techniques that can help you achieve your goals.
Individuals having low self esteem not only find it difficult to understand their feelings but also get involved into various problems and difficulties. In most cases, low self esteem develops at an early age especially because of the surroundings in an around our family therefore, parents should not criticize or scold their child every time they make mistakes. However, this is not the case always, as many adults also develop low self esteem due to family problems, for not getting a proper job, failures, etc. To overcome your problems and face difficult situations there are self esteem self help books describing ways to manage pressure, attend interviews, carry a positive attitude, and make their life stress-free.
Searching the web would help you find many self esteem self help books but choosing the right one is important and essential. Therefore, individuals looking for self esteem self help books should search for books based on their condition and reading reviews about the book they are planning to buy would help them know whether the self esteem self help book they are planning to buy has helped individuals develop self esteem. Having low self esteem would not help you make your life happier therefore it is important for individuals to buy a good self esteem self help book.
Besides there being self esteem self help books there are self esteem self help programs to help you improve self esteem. Individuals should decide which self esteem self help program they would like to learn for boosting self esteem. This is because, there are self esteem self help videos, exercises, courses, books, etc and you should choose a self esteem self help technique that suits your problem and can be followed in the right manner.
It is good to know that different self esteem self help programs have been designed for people having different problems. Find out whether the self esteem self help program would help you overcome low self esteem and provide you the strength to face the difficulties in your life. Moreover, you should choose a self esteem self help program that suits your age and level to help you know tips and techniques accordingly.
Choosing the right self esteem self help technique would teach you to appreciate your talents and value yourself. Although this is one of the basic self esteem technique but this is important and essential.
In spite of popular opinion, construction generally is one of the more stable sectors in the states for those who are wise about it. This is actually the one area which may be safe from economic decline if you carry out things in the correct fashion. It does not matter if the economy is having difficulties, or if there is not a huge upswing in buyer spending ? construction projects are likely to consistently be in development. You really need to be certain that you?re part of the job available.
A primary reason that construction jobs are going to be consistently in progress is the fact that people need places to live and operate. Regardless of whether brand-new residences aren?t being crafted, old ones will most likely be remodeled or removed.
For those on the lookout for tons of employment opportunities, it might be located in the construction sector if you are able to sit and learn a great many assorted skills. Irrespective of the market conditions, consumers and firms will be needing very skilled construction businesses and contractors, in addition to supplies used to build.
Quite a few people believe that the construction industry is linked with the strength or weakness of the country?s economy, but when you offer an unique group of services it is usually simpler to adapt. As an illustration, regardless if brand new construction in the commercial and non commercial areas decrease, one could concentrate on remodeling existing establishments. Consumers and companies are always moving about. This is an chance to help them remodel a spot in order to suit their own preferences. Offering remodeling solutions is a wonderful path to create wealth in construction.
Take into account the scenario of a corporation moving into an existing facility. Whenever they transfer, they could have to remodel and overhaul the inside of the area. For the right independent contractor, this is an chance to generate a nice income doing work that is cherished by the buyer. This is the reason there are several independent remodeling contractors. It usually is quite financially rewarding for those who accurately project ones prices for any undertaking and estimate the proper price.
It could be essential for anyone who is focused on studying this field to review the fiscal rewards which could be realized. There are many reasons to get interested in the most important advantages that may come from working in this field. Folks have made an impressive living utilizing their competence together with wonderful business knowledge. Quite a few construction corporations and general contractors don?t have the greatest organization talents, so making clever judgements and trying to keep expenses low will help you differentiate yourself.
Invest time to investigate if the construction industry is best for you. With the right point of view, you can get work in any economic system. There is no better occasion compared to the present to get involved and become comfortable with the business opportunities that exist to you. Plenty of people have made a cozy living and valued their work as construction laborers.
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KATMANDU, Nepal (AP) ? A plane carrying tourists to view Mount Everest crashed while attempting to land in dense fog in Nepal on Sunday, police and eyewitnesses said. A witness said 18 bodies were pulled out of the wreckage of the plane, which was carrying 19 people.
The Beechcraft-made plane belonging to Buddha Air was carrying 16 foreign tourists and three crew members and crashed in Bisankunarayan village, just a few miles (kilometers) south of the capital, Katmandu.
Police officials said rescuers had reached the crash site, but would not comment on casualties.
An eyewitness, Haribol Poudel, told Avenues Television that the plane had hit the roof of a house in the village and that 18 bodies were pulled out. He said a man who appeared to have survived was taken to a hospital.
Poudel said it was foggy, and that visibility was very low in the mountainous area.
Rewant Kuwar, an official at the Katmandu Airport rescue office, said the plane had last made contact with the control tower at 7:31 a.m.
The plane had taken the tourists to view Mount Everest and other high peaks and was returning to Katmandu. The "mountain flight" takes tourists over the Everest region, and passengers can view some of the world's highest peaks from the airplane windows.
More than the previous few decades, numerous dis-reputable financial institutions have issued loans to borrowers who couldn?t pay for them. The outcome was that these qualities had been foreclosed upon and those financial institutions had been taken more than through the US govt. This has designed a fundamental issue through the government companies that now very own these homes. To irritate the issue, many possible buyers lack the essential money for any down payment.
The answer for the US government was to open up the floodgates and provide federal government owned foreclosures for reduced down payment. In addition to this, special low rate of interest funding can be acquired to buy these properties.
The guidelines for these loans are decreased generating it simpler to obtain a dwelling for those with bad credit score. The US government is even insuring and guaranteeing the residence loans as well as offering approval for borrowers with prior bankruptcy, foreclosure, or brief sale.
VA property financial loan centers within the previous only worked with veterans who?ve served the country in some form of military program. At present, in an effort to promote the economic climate, VA home loan centers are producing obtainable authorities funded subsidized home loan loans for non veterans. The FHA 203k rehabilitation mortgage is supposed to become employed to fix fixer uppers. For these loans you might receive capital for household improvement and in addition to purchase the house. The down payment is often as little as $100. Other govt mortgage products issued by VA HLC include USDA $0 down rural and farm loans.
Looking for the very best property isn?t the first action within the govt foreclosure purchasing approach. It can be pretty critical to not skip the qualification procedure and fill out the VA residence mortgage software prior to searching for homes. The purpose is the fact that most foreclosure bids need pre-approval. It can be essential to get a pre-approval letter immediately from VA HLC or an authorized financial institution. Furthermore to this, VA HLC
All recipients are required to certify that they?ll live inside the home.
Taking advantage from the empty foreclosures inside the area is a great way to develop into a house owner. The VA residence loan program is also open to first time buyers who meet the mortgage qualifications. Stop by or get in touch with your local VA Property Mortgage Center for much more information on obtaining a household through foreclosure.
WASHINGTON ? The world's economic powers struggled on Friday to get on top of a European debt crisis that is threatening to dump the global economy back into recession.
Officials gathered for three days of discussion pledged to push forward to fulfill the goals of a program in which the Group of 20 major economies promised stronger cooperation to jump-start global growth and help Greece avoid a destabilizing default.
But private economists questioned whether the latest action plan unveiled by the G-20 countries Thursday night went far enough to deal with market concerns that a Greek default is a virtual certainty that threatens to destabilize other highly indebted European countries.
All of the discussions about European debt were occurring around the annual meetings of the 187-nation International Monetary Fund and its sister lending agency, the World Bank.
In advance of those talks, the G-20 finance officials, including Treasury Secretary Timothy Geithner and Federal Reserve Chairman Ben Bernanke, pledged a bold effort to deal with the debt crisis and encouraged Europe to move quickly to implement its promises to help Greece.
The G-20 statement had little initial impact on the market's sour mood, with stocks continuing to tumble in Asia. Europe, which had suffered big losses on Thursday, stabilized and managed to eke out small gains on Friday, as did Wall Street. The Dow Jones industrial average was up 0.1 percent to 10,745 while the broader Standard & Poor's 500 index rose 0.5 percent to 1,134.
Private analysts, however, predicted more down days for stocks in coming weeks as investors continue to fear the consequences of a Greek debt default.
Jay Bryson, global economist at Wells Fargo Securities, contrasted the G-20 statement Thursday with the bold program the G-20 put forward in London in April 2009 at the height of that financial crisis with billions of dollars of support put forward to boost economic growth and provide a financial backstop for the IMF to rescue countries in trouble.
"You've got to back up words with actions," Bryson said of Thursday's statement, which he said was an example of "political paralysis."
Sung Won Sohn, an economics professor at California State University's Martin Smith School of Business, said the great concern is that if Greece doesn't make further painful cuts in government spending and ends up defaulting on its debt, the shock waves will rock big banks in France and Europe with heavy exposure to Greek debt and will cause fearful investors to flee the bonds of other heavily indebted countries such as Italy and Spain, countries with much bigger economies.
"The fear in the markets is that the problem will spread to much bigger economies such as Spain and Italy. Europe would not have the resources to handle a crisis of that magnitude," Sohn said.
The finance ministers at the Washington meetings said they believed that the 17 nations that use the common euro currency were getting the message that they needed to move much more quickly to reform their surveillance procedures and boost their economic support.
"The leading lights of the eurozone are aware that time is running out," British Finance Minister George Osborne told reporters on Friday. "There is a far greater sense of urgency than there was three weeks ago about the necessity for the eurozone to address its problems."
Canadian Finance Minister Jim Flaherty said that he had stressed during the G-20 discussions that "Europe will need an exercise of political will. We need decisiveness and we need clarity."
Investors are worried that Europe's debt crisis could destabilize the global economy at a time when growth has already slowed significantly due to a jump in oil prices earlier in the year and a pronounced slowdown in the United States, the world's largest economy.
Greece could default on its debt next month unless it receives a $10.9 billion installment from other eurozone countries and the IMF.
Antonio Borges, the head of the IMF's European department, told reporters Friday that if Greece continues with the austerity measures it has pledged to put in place, then the rescue funds will continue to be provided.
"They can count on full support if they are on track," he said at an IMF briefing. "If they are not on track, then I think they will see the support evaporate very quickly."
A Greek default could destabilize other financially troubled European countries, such as Portugal, Ireland, Spain and Italy. It would also deal a blow to many European banks, which are large holders of Greek government bonds. The concern is that the shock could result in a replay of what occurred when Lehman Brothers fell in September 2009, sending waves of fear throughout the global financial system that caused credit to freeze as banks stopped lending to each other.
Geithner has said the United States has a huge stake in seeing Europe succeed. The G-20 group discussed proposals Thursday that he has raised to expand the resources of the European bailout fund by using methods the United States employed during its own financial crisis in 2008-2009.
The G-20 communique spoke of trying to increase the flexibility of the rescue fund and maximize its resources but spelled out no specific ways to accomplish those goals.
The joint statement also said the G-20 nations planned to produce a "collective and bold action plan" to boost global growth and deal with high government debt that will be put together in time for a G-20 leaders' summit in Cannes, France, on Nov. 3-4. But it provided no details of what will be in the action plan.
G-20 finance officials devoted part of their discussions Friday to the issue of providing more support to poor nations. French Cooperation Minister Henri de Raincourt said that one proposal being examined would be for the rich nations to establish an emergency food stockpile to help poor nations, particularly in Africa, deal with unexpected crises.
He said the stockpile could be tapped quickly during the 90 days it normally takes the international community to organize food shipments to disaster areas. The G-20 officials hope to develop an action plan on development aid to present at the G-20 leaders' summit in Cannes, France.
___
Associated Press writers Gabriele Steinhauser, Harry Dunphy and Luis Alonso Lugo contributed to this report.
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We know that starting at age 40, the levels of the by-product prolactin of testosterone of men increases, stimulating the production of the enzyme 5-alpha reductase that causes the conversion of testosterone to gihydro-testosterones DHT triggering prostate enlargement and Prostate cancer. Therefore in order to prevent the symptoms of enlarged prostate and prostate cancer are to naturally impend the conversion of testosterone to DHT. Unfortunately, aging is not the only causes of enlarged prostate and prostate cancer. In this article, we?ll discuss other causes of prostate enlargement and prostate cancer.
I. Causes of enlarged prostate and prostate cancer
1. Genetics Genetics may also play a role in enlarged prostate since study shows that prostate cancer and enlarged prostates can be inherited from generation to generation.
2. Hormone imbalance The outward appearance of a typical middle-aged person shows increased abdominal fat and shrinkage of muscle mass, which is the main factor causing hormone imbalance. Hormone imbalance is also caused by depression, stress, and anxiety that are the most common psychological complications of hormone imbalance.
3. Cell-growth factor For whatever reason, some people have implicated in enlarged prostate that?s a growth factor.
4. Mineral deficiency Our prostate contains high amounts of zinc to function normally. Zinc deficiency causes the prostate to enlarge. This may be due to aging or uncontrolled diet with excessive alcohol drinking.
One other insightful point concerning this issue. 5. Obesity Study shows that uncontrolled diet that?s high in saturated and trans fats are the main cause of hormone imbalance that results in prostate enlargement. Researchers suggest that excess calorie consumption could somehow directly stimulate prostate enlargement because the excess body fat that accumulates causes an imbalance of hormone levels.
The Pill Causes Breast Cancer and Ella the new Abortion Drug
6. Testosterone Testosterone deficiency may be caused by high amounts of the by-product prolactin of testosterone of men in the body that stimulates even higher productions of the enzyme 5-alpha reductase resulting in a high amount of the conversion of testosterone to gihydro-testosterones DHT thus triggering prostate enlargement. Excess estrogen seems to be the culprit in prostate enlargement that leads to the pathology and favors the development of prostate cancer.
III. Symptoms
1. Nocturia. Nocturia is defined as being awakened at night one or more times in order to pass urine. It becomes more common as the person becomes older. This common pattern seen among people with this condition is an increased number of times of waking up every night to urinate for the rest of their life.
Nocturnal Polyuria is an important cause of nocturia in which there is an overproduction of urine at night. It?s defined to have nighttime urine volume that?s greater than 20-30% of the total twenty-four hour urine volume. Nocturia occurs more commonly among older people. It also occurs in women and men differently. Women generally experience nocturia as a result from childbirth, menopause, and pelvic organ prolapse. Nocturia in men can be directly attributed to benign prostatic hyperplasia (BPH), also known as enlarged prostate.
2. Dysuria. Dysuria is a complication associated with genitourinary infections usually resulting in painful urination because the infection has made its way into the urinary tract, resulting in a urinary tract infection. Dysuria in men is usually indicative of a complication of the prostate, commonly referred to as prostatic hyperplasia caused by hormonal imbalances associated with estrogen production.
3. Asymptomatic As we know the prostate gland becomes enlarged as a man ages. Asymptomatic inflammatory prostatitis may be diagnosed when infection-fighting cells are present causing common symptoms of prostatitis such as difficulty with urination, fever, and lower back and pelvic pain.
4. Epididymitis Epididymitis is an inflammation of the epididymis that connects the testicle with the vas deferens. Epididymitis is usually caused by infection of the urethra or the bladder. Epididymitis may begin with a low grade fever and chills with a heavy sensation in the testicle which becomes increasingly sensitive to pressure or traction.
III. Prevention and treatment
A. With Foods
1. Tomato Tomatoes contain a high amount of lycopene that can help to protect the prostate and keep it healthy, but raw tomato is hard to digest. Be sure to take tomato with dark green leafy vegetables for helping the absorption of lycopene.
2. Soy bean and legume Soybeans and legumes contains isoflavones and is a unique source of phytochemicals It directly inhibits the growth of different types of cancer cells and protects the prostate from being enlarged. Study shows that?soflavones were found to markedly inhibit prostate tumors in mice implanted with prostate cancer cells. Try adding soy or rice milk to your morning cereal or oatmeal.
2. Grapefruit Grapefruits contain high amounts of vitamin C, minerals, bioflavonnoids and other plant chemicals that help to prevent enlarged prostate, prostate cancer and other forms of cancer and heart diseases.
3. Papaya Papayas are rich in antioxidants, vitamin E, vitamin C, vitamin A, carotenoids and flavonoids that have cardio protective and anti-cancer effects. Eating papayas help to promote lung health and prevents the prostate from becoming enlarged and prevents prostate cancer.
4. Bean Beans are rich in inositol pentakisphosphate. Beans are high in fiber, which helps the body rid itself of excess testosterone that reduces the conversion of testosterone to gihydro-testosterones DHT thus triggering prostate enlargement and other problems.
5. Green tea Green tea contains the antioxidant phytochemicals called polyphenols that attack growth factors and proteins, interrupting processes that increase the size of tumors, thus preventing them from spreading to other parts of the body. Green tea also help to prevent the development of benign prostate hyperplasia such as enlarged prostate and prostate cancer.
6. Broccoli Broccoli and other cruciferous vegetables, including kale and cauliflower, are rich in sulphoraphane, a cancer-fighting phytochemical that helps rid the body of excess testosterone and reduces the risk of benign protatic hyperplasia and prostate cancer.
7. Cold water fish Cold water fish contains high amounts of Omega-3 fatty acids. Study shows that omega-3 fatty acids inhibit carcinogenesis that helps significantly lower levels of omega-3 polyunsaturated fatty acids in men with benign prostate hyperplasia and prostate cancer.
B. With Supplements
1. Bee pollen Bee pollen is an antioxidant containing high amounts of flavonoids, zinc and many other elements needed by our bodies. It has an astounding record at reversing prostate cancer and other prostate problems such as enlarged prostate.
2. Prostate glandulars Animal prostates contain high amounts of zinc that nourishes the human prostate.
3. Alanine Since alanine is present in prostate fluid, it plays an important role in prostate health. This has been confirmed in some studies that Alanine helps in preventing enlarged prostate and helps in strengthening the immune system.
4. Glutamics acid The fluid produced by the prostate gland also contains high amounts of glutamic acid, and may play a role in the normal function of the prostate. High dosages of glutamic acid may cause headaches and neurological problems and people with kidney or liver disease shouldn?t consume high intakes of amino acids without first consulting with your doctor.
5. Glycine Glycine is also found in fairly large amounts in the prostate fluid and may for this reason be important in prostate health. It?s required to build proteins in the body and synthesis of nucleic acids, the construction of RNA as well as DNA, bile acids and other amino acids in the body. Study shows that glycine, taken withalanine and glutamic acid help to reduced the amount of swelling in the prostate tissue.
6. Lycopene The substance found in tomatoes, fruits and vegetables can prevent and slow down prostate inflammation and prostate cancer. Study shows that lycopene helps to decrease in DNA the damage to prostate cells. Lycopene also protects against LDL cholesterol oxidation.
C. With Herbs
1. Saw palmetto Saw palmetto is the number one choice when it comes to prostate health. Recent study of American Society for Cell Biology shows that saw palmetto inhibited prostate cancer cell growth in the test tube. It has been used for a long time as an herb to treat an enlarged prostate gland in men. It may also be used to treat prostate cancer. This herbal remedy has no significant side effects and only rarely causes mild stomach upset or diarrhea.
2. American Ginseng The roots, has been used for centuries in Asian medicine as an aphrodisiac, a tonic for well being and a curative. American Ginseng is helpful in treating prostate problems due to low testosterone and androgen levels. It also inhibits cancer cells proliferation in a variety of cancer such as lung cancer, colon cancer and prostate cancer.
3. Corn silk Corn silk contains certain therapeutic properties that help to relieve the inflammation of the prostate. Corn silk are also popular as an alternative to standard Western allopathic medicine for treating bed-wetting and obesity.
4. Pumpkin seed extract Pumpkin seeds contain high amounts of zinc and fatty acids which is necessary for the prostate?s health. Pumpkin seed extract helps to tone the bladder muscles, relax the sphincter mechanism and recongest the prostate.
5. Pygeum Africanum Pygeum Africanum is rich in phytosterols, the substances that inhibit the biosynthesis of prostaglandins, which is the process responsible for prostate inflammation. Phytosterols also help to reduce the size of the prostate, promote normal urination and reduce cholesterol deposits in the prostate that can occur with BPH.
6.Essiac Essiac is useful to regulate prostate gland function, stimulates gland activities, promote circulation of blood, relieves pain and inflammation and may permit increase sexual activities. Study shows that essiac possesses potent antioxidant and DNA-protective activity, properties that are common to natural anti-cancer agents.
7. Uva ursi Uva ursi is an evergreen perennial shrub. It has been used since the year 150AD by various civilizations to treat urinary tract infections, kidney problems, Prostate, and bladder dysfunction.
8. Soy isoflavones Study shows that an increased soy intake is related to a reduced risk of prostate cancer. Vitro tests showed that soy isoflavone inhibits growth of both androgen-sensitive and androgen-independent prostate cancer cells.
D. With Chinese Herbs
1. Saw palmetto Saw palmetto has been used for over a century in traditional Chinese medicine in treating pain in the lower back, inflammation and enlargement by inhibiting dihydrotestosterone, thereby reducing its stimulation for cancerous cell multiplication.
2. Patrinia (Bai jiang cao) Patrinia helps the body get rid of prostate inflammation and damp heat that exists in the body. In Chinese medicine damp heat in the prostate could be caused by bacteria infection, drugs and other conditions such as a habit of eating hot, spicy or greasy foods.
3. Lu lu tong (liquid amber) Lu lu tong has the ability to improve qi and blood circulation . It also helps to reduce the abdominal, back and knee pain caused by damp heat as well as difficult urination because of bladder or prostate inflammation.
4. He shou wu He shou wu contains several derivatives of tetrahydroxystilbene. These antioxidants and anti-inflammatory compounds may act as an estrogen, reducing levels of circulating male hormones such as DHT that fuel the growth of prostate cancer.
5. Niu xi (Achyranthes) Niu xi contains triterpenoid saponins and sitosterol that possesses anti-inflammatory effects in both enlarged prostate and prostate cancer inflammation. It also helps to nourish the kidney liver and reduce symptoms of damp heat and difficult urination as well as stiffness and pain of lower back.
6. Gui Zhi (tokoro) Gui Zhi is used for urinary tract disorder that pertains to ying qi levels which is the main cause of prostate inflammation. It also is used as a tonic and blood purifier.
7. Astragalus root Astragalus root is a sprawling perennial legume. The Chinese medicine uses the dried sliced or powdered root of the plant to enhance immune function by increasing the activity of certain white blood cells, which increases the production of antibodies. It also helps to increase the body?s resistance to infections, to heal the allergies, and to raise and renew the vitality.
8. Che Qian zi (plantago seed) it?s mainly used for stone strangury caused by lower burner damp-heat, such as the symptoms of aching pain in the lumbus and abdomen, poor urination or with hematuria, and urinary tract stones.
9. Vaccaria seed it?s used to reduce pain and stiffness in the lower back, drain excessive damp heat, invigorate blood and treat difficult urination.
E. With Vitamins & Minerals
As we mentioned in previous articles, We know that starting at age 40, the levels of by-product prolactin of testosterone of men increases, stimulating the production of the enzyme 5-alpha reductase that causes the conversion of testosterone to gihydro-testosterones DHT triggering prostate enlargement and other problems. Besides aging there are many other causes of enlarged prostate and some of them might result in prostate cancer. Beside foods nutrition and herbs, vitamins and minerals also play an important role in preventing and treating prostate enlarged as well as prostate cancer.
1. Zinc Zinc is necessary for male sexual and prostate health. Zinc inhibits the uptake of testosterone into prolactin. The prostate gland contains a higher concentration of zinc than any other organ in the body, therefore zinc deficiency is the major cause of enlarged prostate as well as an early indication of prostate cancer.
2. Selenium Selenium contains an antioxidant enzyme that helps to enhance immune function, stop early cancer cells in their development and control cell damage that may lead to cancer. Foods that contain high levels of selenium are bran, broccoli, eggs, mushroom, and basil nut.
3. Vitamin C As we know Vitamin C, a water-soluble vitamin, is commonly known as an antioxidant. Some studies show that increased levels of vitamin C reduce the risk for prostate cancer as well as prostate inflammation and enlarged prostate.
4. Vitamin D Vitamin D is found in foods and is also produced by the body and activated after exposure to ultraviolet light from the sun. Studies show that activated vitamin D may be broken down in the prostate, where it may plays an important role in preventing enlarged prostate and prostate cancer. Vitamin D deficiency may increase the risk of prostate cancer.
5. Vitamin E Vitamin E plays an important role in preventing prostate cancer because it interferes with prostate specific antigen (PSA) and androgen receptor proteins that play a central role in the development of the disease.
F. With Homeopathic Remedies
1.Chimaphilia Chimpaphilia acts principally on kidneys, and genito-urinary tract; affects also lymphatic and mesenteric glands. It helps in treating urge to urinate with burn pain, strain to urinate and prostate enlargement and irritation.
2.Conium maculatum Conium maculatum is an excellent remedy for old age people. It helps to improve prostate enlargement, difficult urination, weakened urine flow and soften the prostate and enhance sexuality.
3. Hepar sulphuris calcareum Hepar sulphuris calcareum is a mixture powder of oyster shells and pure flower of sulfur. You can purchase in mixture in a health food store and follow the instructions to make your own. Hepar sulphuris calcareum has proven record in treating dribbling urine and poor flow.
4. Sabal serrulata Sabal serrulata is homeopathic to irritability of the genito-urinary organs. It?s helpful in treating difficult urination caused by enlarged prostate, constant desire to urinate, as well as sexual inability.
5. Staphysagria Staphysagria is helpful in treating diseases of the genito-urinary tract such as the urge to urinate, inability to urinate fully and feeling of bladder not being empty.
6. Thuja The main action of thuja is on the genito-urinary organs. It?s helpful in treating enlarged prostate, sudden desire to urinate and split urinary stream.
G. With Common Sense Approaches
1. Reduce fat and cholesterol intake, especially saturated fats. Study shows that if you eat more than five servings of red meat every week you could increase the risk of enlarged prostate and prostate cancer by 80%. By eating less of red meat and replacing them with cold water fish, you?re ensured yourselves to get enough omega fatty acid that helps to improve your prostate health, reduce cholesterol levels, lower the risk of enlarged prostate as well as prostate cancer.
2. Eat foods containing more zinc (nuts- especially walnuts, pumpkin, seeds, safflower seeds and oysters). Zinc is an essential mineral for prostate health. It helps to reduce the size of the prostate and relieve symptoms of enlarged prostate. Foods containing zinc also contain cucurbitacines, chemicals that stop testosterone from changing to stronger forms of testosterone which encourages too many prostate cells to grow. Foods that contain cucurbitacines are nuts, walnuts, pumpkin seed, and oyster.
3. Guard your prostate with green tea Green tea contains catechins, a chemical that helps our immune system to track down tumor cells before they can do any harm to the prostate cells. Green tea may be one reason that Asian people have far fewer cases of prostate cancer than North Americans.
4. Remove all chemicals and pesticides from diet (eat organic food). Chemicals and pesticides in the processing food is toxic to our body. Intaking high doses of these poisonous chemicals will danger our immune system as well as cause inflammation of the body including the prostate.
5. Increase consumption of plants high in the sulphurophanes Study shows that you could reduce the risk of prostate cancer and prostate enlargement by 45% if you eat twenty-four or more servings of vegetables that contain high amounts of sulphurophanes such as broccoli, cauliflower, brussel sprouts, and cabbage.
H. Activities That Help to Maintain a Healthy Prostate
1. Moderate exercise (like walking) We know that one half to three-quarters of men older than seventy-five will have some cancerous changes in their prostate glands. People doing moderate exercise like walking for two to three hours per week had a twenty-five percent less risk of developing prostate problems than those who didn?t. Just 20-30 minutes walking a day would help to increase circulation of blood to the body as a side benefit for better sexual function and prostate health.
2. Sitz baths it?s one of the earliest prostate treatments and the most effective remedies for prostate pain. This special bath brings a great flow of blood to a specific area of the body while the regular hot bath is pleasant and generally increases circulation throughout your body.
3. Yoga Yoga is really helpful to the prostate. It also helps tone the deep pelvic muscles there by promoting circulation. Yoga promotes flexibility, circulation, and stimulates the liver meridian. In general yoga helps a person to be more aware of his pelvic area in terms of his muscular control.
4. Kegal exercise Kegal exercise when performed while in virasana, bhekasana or frog pose also promotes good prostate health and overall benefit to the urogenital tract.
5. Spinal and pelvic massage Spinal and pelvis massage helps to a) Increase blood supply to the prostate and the pelvic area. B) Increase lymphatic flow to the prostate and the pelvic area. C) Reduces inflammatory conditions.
6. Joyful sex life Sex helps increase movement of the pelvic muscles and the blood circulation to all organs of your body. As fresh blood supply arrives your cells, organs and muscles are saturated with fresh oxygen and hormones, and as the used blood is removed, you also remove waste products and toxins from your body, thus reducing the risk of inflammation of the prostate.
You can see there are many different methods that help to maintain a healthy prostate. I hope this information will help. If you need more information. Please visit my home page at:
Kyle J. Norton
[http://medicaladvisorjournals.blogspot.com/]
http://prostatehealth09.blogspot.com/
All rights reserved. Any reproducing of this article must have all the links intact.
I?ve been studying natural remedies for disease prevention for over twenty years and working as a financial consultant since 1990
Fly over any baseball stadium when the home-team batter slams a double in the gap with two men on base, and you?ll see a crowd of fans rising in unison, arms waving wildly in the air. You?d think you were viewing typical baseball fan behavior.
Witness this scene at ground level, though, and you?ll get a different picture. While a majority of fans participate in the cheering, others are sipping beer, attending to scorecards or roaming the walkways in search of a hot dog.
Such displays of individuality shouldn?t come as a surprise ? people often react differently to the same circumstances, after all. But life?s individuality thrives at much smaller scales than the human body.
Cells, for example, are not all alike. Clusters of single cells, even of all the same type, show cell-to-cell differences in appearance, growth and behavior. This holds true for single-celled organisms too. Individuals within a colony of identical bacteria, for instance, will behave in different ways, even under the same conditions.
For decades, scientists were stuck with an aerial view of cell behavior. Experiments carried out on large pools of cells obscured information on the activities and makeup of the individuals. Difficulties in tracking molecules inside a single cell forced researchers to content themselves with averages derived from whole populations.
Now scientists are finding ways to zero in. Improvements in imaging techniques make it possible to view tiny, transparent structures and molecules within cells that are otherwise nearly invisible. New ways of separating cells during study enable scientists to sort through thousands at once, distinguishing subpopulations and studying multiple traits in just one go.
?We used to think that we could take a million cells and grind them up and make measurements on those million cells,? says Nancy Allbritton, a biomedical engineer at the University of North Carolina at Chapel Hill. ?And whatever measurements we made on those million cells would reflect what?s going on in a single cell.?
Scientists today know that averaging the constituents of a million cells often gives no clue to what?s going on at the cellular level. Cells thought to be alike have been found to produce different numbers and types of proteins. Beyond providing interesting insights into basic biology, understanding this individuality may have medical consequences ? illuminating characteristics that can make a cell more amenable to a particular drug or more vulnerable to an allergen or viral infection.
Though in many ways single-cell analysis is still in its infancy, the field is quickly expanding. Researchers are working to improve technologies and to figure out how to make sense of the data.
Lighting the way
Of the millions of different living things in the world ? large and small, plants, animals and microbes ? all have in common the fact that they are made of one or more cells. Scientists have been observing cells since the mid-17th century, when Dutch scientist Antony van Leeuwenhoek discovered bacteria and, later, blood cells. Through the years, microscopes and staining methods have allowed researchers to view and describe the basic structures and internal workings of various cell types ? understanding how skin cells, for example, differ from cells in the heart or kidneys.
Distinguishing unique features among cells of the same type has proven to be more difficult, because cells are so small. A typical human cell is about one one-hundredth of a millimeter across. Microbes are even smaller. Conventional light microscopes are constrained by a physical law demanding that the size of an object being imaged be no smaller than about half the wavelength of the light used to produce that image. To see anything as small as a molecule within a cell (the scale at which much of the work gets done), some tool other than traditional light microscopy is needed.
Scientists have found ways to get around this blind spot. When molecules and proteins are labeled with fluorescent dyes, the components inside a cell appear as bright colored dots against the background of a cell?s gel-like filling. Such advances have allowed researchers to see cellular processes unfolding at nano?meter scales. Fluorescent probes have also brought to light the wide variation that exists within a population of seemingly alike members.
When sorting through a population of cloned lung cancer cells loaded up with fluorescent probes, systems biologist Steven Altschuler noticed that each cell carried its own combination of the molecules that send signals between cells.
?It was a surprise to us,? says Altschuler, of the University of Texas Southwestern Medical Center at Dallas, who jointly runs a lab with pharmacologist Lani Wu. ?It even made us wonder what the definition of a clone was.?
The team developed a way to combine fluorescent microscopy with a type of software designed for face recognition to look for patterns among the cells. The team first labeled the cells by attaching different fluorescent tags to proteins involved in cell signaling. Computers then analyzed pictures of the cells, pixel by pixel, looking for variation in the location and intensity of the molecules. The scientists also trained the computers to identify combinations of markers that commonly appear together ? discovering the cellular equivalent of a ?blue-eyed blond.?
Using this method, the scientists were able to identify small subpopulations of cells, or stereotypes, based on which molecules appeared and where. Exposing the stereotypes to the anticancer drug Taxol revealed that different subpopulations respond differently to the drug. In May 2010 in Molecular Systems Biology, the team reported on how individual cell differences could help predict the outcome of treatment.
?That?s definitely where we would like to go,? Altschuler says. ?The dream is that you would be able to look at a small number of cells from a cancer tumor and try to predict the optimal combination of drugs to treat that particular tumor.?
Though doctors currently use various profiling techniques to determine the best treatment, Altschuler says this new approach differs because, in principle, it would allow a doctor to look at every cell in a tumor biopsy, identifying clues that would get lost in a ?population averaging? approach.
Multiplying traits
But fluorescent microscopy has its own limitations. Scientists can look for only a handful of variations, or biomarkers, in a cell at one time. Though cells carrying biomarker A could be distinguished from those carrying B or C, for example, scientists can?t simultaneously scan the cells for a second characteristic that might be important in understanding the first.
Altschuler and Wu?s team has found ways to maximize the use of fluorescent probes to get more details on cell-to-cell differences. Using three out of four available probes to home in on subpopulations of interest, a fourth probe can be free to search for a second variation within each of those groups. By running a series of experiments, rotating the fourth marker?s target each time, scientists can get more detailed information on members in each group.
Say you?re trying to understand people at a baseball stadium, instead of cells, and you want to figure out differences in the way the players, vendors and fans dress. Three out of four fluorescent probes would be used to separate people into each of these groups. The fourth probe could then search for a specific item, such as sneakers, which may be worn by members in each group. In subsequent experiments, the first three probes remain set to identify players, vendors and fans, while the fourth spots people sporting home-team jerseys, then baseball caps and so on. A computer program stitches together the information gleaned from the serial studies to show what people in each group wear.
In this process, a scientist will never look at the jerseys and caps on any given person at the game, because only one measurement can be made at once. ?Still, reliable information can be gained,? Altschuler says. His group is now profiling cells in this way.
At her University of North Carolina lab, Allbritton and colleagues are developing ways to study multiple variables beyond the limitations of microscopy. By employing what they call ?separation techniques,? the researchers have the potential to measure the activity of many enzymes ? a dozen or so ? at once. Each cell gets its own well in a grid containing thousands of rows and columns.
Cells are first loaded with various enzyme substrates, substances that work with an enzyme to produce a specific reaction. The cells are then killed and their contents released. By separating out each of the chemicals that were put into the cells as well as the products the cells make, scientists get a readout on what?s going on inside.
Because each cell sits in its own well, scientists can analyze the cells separately. Comparing the reactions of different cells is key, Allbritton says, because a study of a single cell won?t reveal much variability.
?That?s like looking at one human and trying to understand how variable people are,? she says. ?You would assume if you got a redheaded person that everyone had red hair ? and was 5-foot-4 and was female.?
Allbritton is using this technique to measure protein activity in human cells. Her studies currently focus on kinases, proteins that play a vital role in cell signaling and the regulation of gene activity.
Live on and inform
Such clues to what?s going on inside single cells often come at a price: Cells are damaged or killed in the process, providing only a single snapshot in time. This makes it hard to predict how a single cell will respond to a given stimulus.
J. Christopher Love, a chemical engineer-turned immunologist at MIT, combines the idea of separating cells and the helpfulness of fluorescent dyes to follow biochemical processes over time in living cells. His lab takes an array of subnanoliter-sized wells and fills each with either a single immune cell or a small group of them. With tens of thousands of wells, the unit looks like an ice cube tray the size of a microscope slide. Fluorescent markers identify the various proteins in or on each cell, distinguishing one cell type from another.
Over the course of several hours or days, Love studies the proteins and chemicals secreted by the cells to see how they change. Borrowing a technique from the printing of U.S. dollar bills, he presses the microarray against a glass plate to get a printout (so to speak) of the proteins and chemicals secreted by the cells. Unlike Allbritton?s work, the method doesn?t kill the cells because information is captured from the outside, on the glass plate.
Love is applying this interrogation method to ask questions that are relevant to chronic diseases such as HIV, autoimmunity, cancer and allergies. One ongoing study tracks how allergic patients become desensitized to milk allergens, measuring how the patients? white blood cells respond to milk extracts. The study looks at how many cells are responding, what kinds of immune proteins are being produced and how much of each protein is produced by each cell.
By analyzing large numbers of single events and interactions between small groups of cells in concert, Love can spot rare types of cells and events that are linked to disease. Certain T cells and B cells found in diseases like multiple sclerosis or diabetes, for example, typically are present at rates of 1 in 10,000 to 1 in 100,000 of the circulating T cells in a blood sample, he says. Without looking at many, many cells, he wouldn?t be able to spot or study the interactions of these rare types.
?Single-cell analysis is a bit of a misnomer in the sense that you?re not really looking at one cell,? Love says. ?You?re resolving to one cell, but you are needing to look at thousands, if not tens or hundreds of thousands, of cells to understand what that individual measurement looks like in relation to the rest of the distribution.?
Single microbes
Studies of cell-to-cell differences also promise to reveal new information about single-celled organisms. For a long time, biologists thought that members of a microbe colony were all basically the same, and that those cloned from a single cell, because they were genetically identical, should react to environmental challenges in the very same way.
Scientists now know that individual traits may, in fact, serve as drivers to boost the robustness and resistance of microbial populations, says Michael Konopka, a biochemical engineer at the University of Washington in Seattle. In the October 2010 issue of Nature Chemical Biology, he and coauthor Mary Lidstrom outlined how some members of a microbial colony will stop growing in times of stress, moving into a dormant state that allows them to ride out the bad conditions while their neighbors perish.
Understanding how individuals adapt to environmental conditions, such as temperature fluctuations, could help engineers find ways to boost certain types of chemical processes used in manufacturing, Konopka says. Some naturally occurring microbes, for example, may have a built-in capacity to do the chemical reactions that industrial researchers want to perform.
Others research teams are developing ways to determine the complete genetic blueprint of single-celled organisms to get insight into whole populations of cells that are otherwise difficult to study. Stanford University scientists working under the direction of Stephen Quake are plucking single cells from populations found in pond scum, soil or even plaque on teeth, to ask a seemingly simple question: Who are you?
Conventional genetic analysis techniques require a large, pure sample of the microbes, says Paul Blainey, a postdoctoral researcher in Quake?s lab. ?That usually requires that you?re able to isolate the particular organism and grow up a whole huge beaker of it in the lab.? Unfortunately, the chemical reaction used to amplify tiny amounts of DNA is notoriously prone to contamination.
To get around this problem, the Stanford group is using a laser tweezer to sort individual microbes inside tiny, automated devices designed to analyze minute traces of DNA. Enclosed within this miniature lab, microbes and their DNA are safe from contaminants.
In 2007, Quake?s group analyzed the genome of a single bacterium found in dental plaque taken from a person?s mouth. This year the team turned to a less familiar class of single-celled organisms, the Archaea: Online February 22 in PLoS One, the researchers published findings on the genome of a single-celled ammonia-eater from San Francisco Bay.
Genetic information gleaned from an individual microbe, combined with findings on cell-to-cell differences in behavior, may provide new insights into the thousands of unknown or barely known populations found in water and soil. Such insight could in turn offer clues to how microbes act as infectious agents and how they develop resistance to antibiotics.
Despite recent gains in studying single cells and how they differ, there?s much work to be done, Allbritton says. Current techniques still allow only a small number ? fewer than two dozen ? of the thousands of molecular components that float around in cells to be measured at one time.
?It?s embarrassing,? she says. ?We?ve made a lot of progress, but you can see how far we have to go.?
With such a small subset of cellular components under study, scientists? perception of how cells operate may be colored by what they see, she adds. The big push now is to develop ways to measure more of the cell?s contents, and see how they change over time and work in relationship to each other.
?If we can see everything,? Allbritton says, ?it might dramatically change how we view the single cell.?
